Abstract
Seven Papilionoideae species ( Eriosema shirense, E. ellipticum, E. pauciflorum, E. gossweilleri, E. psoraleoides, Adenolichos anchietae and Droogmansia megalantha), the roots of which are traditionally used in the South of Angola for handmade soft dairy products, were studied in order to identify new milk clotting enzymes for industrial use. In general, root and leaf extracts showed higher proteolytic activity than stem, fruit or inflorescence extracts. The root and leaf extracts were screened for clotting activity and curd strength. The highest proteolytic specific activity was observed in leaf extracts of D. megalantha and the lowest clotting time was obtained for leaf extracts of A. anchietae and E. psoraleoides. When stored at −20°C for 2 months the extracts lose 16–40% of proteolytic activity and its clotting time increased 10–40%. Only the extracts from E. psoraleoides dried leaves retain clotting activity (90%) after 4 years storage at −20°C.
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