Abstract
The present study examined the potential antinociceptive activity of C18 5-HT (βN-octadecanoyl-5-hydroxytryptamide) using chemical and thermal nociception models in mice. Orally administered C18 5-HT (0.1, 1 and 10 mg/kg) produced significant dose-dependent antinociceptive effects in formalin-, capsaicin- and glutamate-induced licking models. This compound also induced a significant increase in the response to thermal stimuli in the hot plate test, and its antinociceptive effect was not related to muscle relaxant or sedative actions. In a thermal hyperalgesia model, C18 5-HT presented an anti-hyperalgesic profile as evidenced by the increase in the response time of the animals. Furthermore, intraperitoneal (i.p) pretreatment with naloxone (a non-selective opioid receptor antagonist, 1 mg/kg), ondansetron (serotoninergic receptor antagonist (5-HT3 subtype), 0.5 mg/kg) or AM241 (CB1 cannabinoid receptor antagonist, 1 mg/kg) reversed the antinociceptive effects of C18 5-HT in the hot plate model. In the formalin-induced licking model, pretreatment with naloxone reversed the antinociceptive effects of C18 5-HT, as demonstrated by an increase in the paw licking response when compared with the C18 5-HT-treated group. These findings suggest that C18 5-HT has peripheral and central antinociceptive effects and that its mechanism of action involves, ate least in part, opioid, serotoninergic and cannabinoid pathways.
Highlights
The present study examined the potential antinociceptive activity of C18 5-HT using chemical and thermal nociception models in mice
To investigate if the participation of glutamatergic system was involved in C18 5-HT antinociception, we evaluated whether pretreatment with this substance would reverse the antinociceptive effects associated with the intraplantar glutamate injection
The present study demonstrated, for the first time, the antinociceptive effects produced by βN-octadecanoyl5-hydroxytryptamide (C18 5-HT)
Summary
The present study examined the potential antinociceptive activity of C18 5-HT (βN-octadecanoyl-5hydroxytryptamide) using chemical and thermal nociception models in mice. In the formalin-induced licking model, pretreatment with naloxone reversed the antinociceptive effects of C18 5-HT, as demonstrated by an increase in the paw licking response when compared with the C18 5-HT-treated group. These findings suggest that C18 5-HT has peripheral and central antinociceptive effects and that its mechanism of action involves, ate least in part, opioid, serotoninergic and cannabinoid pathways. The purpose of the present study was to investigate the antinociceptive effects of C18 5-HT using selected chemical (formalin-, capsaicin- and glutamate-induced licking response) and thermal model of nociception in mice. Statistical significance was calculated by two-way ANOVA followed by Bonferroni’s test. *Indicates p < 0.05 when compared to the vehicle-treated mice
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