Abstract
The present study focuses on possible ways to protect brain neurons during neuroinflammation. For the first time it is shown that peptide NPNDKYEPF amide, similar to activated protein C (APC), protects hippocampal neurons in a model of neuroinflammation induced by the toxic effects of endotoxin (lipopolysaccharide)-activated mast cells on neurons. It was found that the incubation of hippocampal neurons with mast cells activated by proinflammatory factors leads to neuronal apoptosis within 24 h after the exposure. Preincubation of mast cells with peptide NPNDKYEPF amide or with APC, before the toxin’s treatment, abolishes the toxic effects of the activated mast cells on neurons. By the blockade of protease-activated receptors of type 1 (PAR1), the receptor mechanism of the peptide action on mast cells and on neurons was identified. It was shown that PAR1 is required for the protective effect of the peptide in the conditions of neuroinflammation. Thus, peptide NPNDKYEPF amide is a neuroprotector, similar to APC, and can be used for the development of new approaches of the therapy of inflammatory processes accompanying different types of traumatic and ischemic brain damage.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
