Abstract
Uric acid (UA) and folic acid (FA) are compounds of biomedical interest. In humans, about 70% of daily uric acid disposal occurs via the kidneys, and in 5–25% of humans, impaired renal (kidney) excretion leads to hyperuricemia. Folate is another form folic acid of which is known as, is one of the B vitamins. It is used as a supplement by women to prevent neural tube defects developing during pregnancy. Polyortho-methoxyaniline nanostructures (POMANS) was synthesized with a new two phase (organic-water) synthesis method. The POMANS was characterized using transmission electron microscopy (TEM) and Fourier transform IR (FTIR). This polymer was used to construct a modified multi-wall carbon nanotube, graphite paste electrode (POMANS-MWCNT/GPE). Linear sweep voltammograms (LSV), cyclic voltammetry (CV) and chronoamperometry were used to investigate the suitability of polyortho-methoxyaniline with multi-wall carbon nanotubes paste electrode as a modifier for the electrocatalytic oxidation of UA and FA in aqueous solutions with various pHs. The results showed that POMANS-MWCNT/GPE had high anodic peak currents for the electrooxidation of UA and FA in pH6.0.Under the optimized conditions, The catalytic peak currents obtained, was linearly dependent on the UA and FA concentrations in the range of 0.6–52 and 0.5–68μM with two segments and the detection limits 0.157 and 0.113μM for UA and FA were, respectively. Finally, the proposed method was also examined as a sensitive, simple and inexpensive electrochemical sensor for the simultaneous determination of UA and FA in real samples such as urine and serum.
Highlights
Conducting polymers (CPs) such as polyaniline, polypyrrole, polythiophene and their derivatives, has been used as modifier in electrochemical sensors, catalysts and electro chromic materials and many more, etc. [1,2,3,4,5]
The morphology of poly ortho-methoxyaniline nano structures was characterized by transmission electron microscopy and Fourier transform IR
The voltammetric investigations were performed in the pH range of 4.0–8.0 using 0.1 M phosphate buffer solution (PBS) as supporting electrolyte, containing 25 μM Uric acid (UA) and 50 μM folic acid (FA) at scan rate 20 mV s−1(Fig. 3a).As can be seen in Fig. 3b & c, the anodic peak potential shows a negative shift by increasing the solution pH with a slope value of 45.0 mV and 59.0 mV per pH unit for UA and FA, respectively.From the results, it can be concluded that equal numbers of electrons and protons are involved in the electro-oxidation of UA and FA on the surface of the Polyortho-methoxyaniline nanostructures (POMANS)-MWCNT/GPE [13,18]
Summary
Conducting polymers (CPs) such as polyaniline, polypyrrole, polythiophene and their derivatives, has been used as modifier in electrochemical sensors, catalysts and electro chromic materials and many more, etc. [1,2,3,4,5]. UA and FA always co-exist in the human body fluids It has essential for the simultaneous determination of UA and FA. It has been reported that carbon nanotube-modified electrodes were successfully applied to study and determine many biological compounds, drugs and toxic materials [25,26,27,28]. There are no similar studies on the polymerization of poly ortho-methoxyaniline nanostructures (POMANS) and its application as novel modifier in modified graphite paste electrode including MWCNTs for the simultaneous determination of uric acid and folic acid in aqueous solution. In order to demonstrate the catalytic ability of the modified electrode in the determination of UA and FA in real samples, we examined this method for the voltammetric simultaneous determination of UA and FA in samples of serum and urine
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