Abstract
The purpose of this study is to establish a novel method for the detection of mitochondrial DNA (mtDNA) polymorphism based on denaturing high-performance liquid chromatography (DHPLC) and to explore the new mitochondrial DNA polymorphisms in coding region in order to improve the discrimination power of mtDNA in forensic DNA typing. MtDNA coding region loci were amplified by the polymerase chain reaction (PCR). Using a technique of DNA pools and performing the analysis of pairwise combining samples, two strategies that based on the most homologous and retention times of cartridge were evaluated and optimized. The new strategies were the foundation for exploring polymorphism in entire mitochondrial genome and were expected to be important methods for forensic mtDNA identification.
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