New steroidal saponins from the aerial parts of Paris polyphylla var. yunnanensis and their effects on blood coagulation

Abstract

Five new steroidal saponins, paripolins D-H (1–5), and 6 known compounds (6–11) were isolated from the aerial parts of Paris polyphylla var. yunnanensis. The structures of 1–5 were determined using spectroscopic analyses in conjunction with acid hydrolysis. It is for the first time to report the 12-hydroxysteroidal saponins from the genus Paris. The effect of all isolated compounds on blood coagulation was determined in vitro using the plasma recalcification time method. Compounds 1 and 2 showed potent procoagulant activity, and 5–11 exhibited significant anticoagulant activity.