New routes to the preparation of silver-soft liner nanocomposites as an antibacterial agent

Abstract

This study evaluated the antimicrobial and physical properties of silver-soft liner nanocomposites (Ag-SLN). Polymerized acrylic soft denture liner disk specimens containing 0 (control), 1500, 3000, or 6000mg/L of silver nanoparticles were placed on the flat bottom of the separate 12-well cell culture plate dish and 100μL samples of microbial suspensions of Escherichia coli strain were inoculated on each specimen and incubated at 37°C, for 24 and or 72h. The antimicrobial effects were determined according to the number of viable cells in the retrieved suspension. Characterization of silver nanoparticles was carried out based on UV–vis spectroscopy and transmission electron microscopy (TEM) analysis. The specimens were characterized by thermal gravimetric analysis (TGA), field emission scanning electron microscope and energy dispersive X-ray analysis (FE-SEM/EDAX), and atomic absorption spectroscopy (AAS). We successfully prepared Ag-SLN and identified the great excellent antimicrobial activity for E coli.