Abstract
A method is described which allows the combination of high-performance liquid chromatography (HPLC) and electrospray ionization mass spectrometry for the analysis of nucleotides and oligonucleotides without compromising the performance of either technique. The essential feature of the method uses 1,1,1,3,3,3-hexafluoro-2-isopropanol as an additive to the HPLC mobile phase. This novel additive results in both good HPLC separation and efficient negative ion mode electrospray ionization. The method is demonstrated for oligonucleotides samples such as synthetic homopolymers of thymidine (PolyT), of fragments based on the pBR322 plasmid sequence and analysis of phosphorothioate ester antisense oligonucleotides. The differentiation of phosphorylation states of natural nucleotides as well as nucleotide analogues, such as ziduvodine (AZT), is also demonstrated.
Published Version
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