Abstract
Abstract In this paper, we report an eco-friendly approach for the removal of 4-chlorophenols from wastewater, through the use of horseradish peroxidase (HRP) immobilized on GO_Fe3O4/Au@CA nanoparticles. The effects of coupling time and enzyme concentration on the immobilization efficiency were investigated. The effects of reaction time and the reusability on the removal of 4-chlorophenols were also evaluated. In the first 30 min, the degradation process promoted by immobilized HRP was fast and increases up to 67%. The degradation of 4-CP in the presence of immobilized HRP was as high as 98% after 180 min. It is worth noticing that, after 180 min the TOC% removal was 85%. The immobilized HRP retains 95% of the initial activity for the first three cycles. These very high performances were promoted by gold, which contributes significantly to the excellent catalytic activity of our nano-catalyst.
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