Abstract
AbstractSnow mold fungi cause serious damage to the seedlings of some coniferous species in the boreal region where deep snow covers the seedlings in winter.Racodium therryanumis one of the fungi causing such damage in Japan. Neither sexual nor asexual spores ofR. therryanumhave been found in the field, and thus, the taxonomy and life cycle of this fungus is unclear. In this study, we developed seven microsatellite markers for population studies such as population structure, recombination and genetic diversity ofR. therryanumthrough de novo genome assembly. Seven microsatellite markers were developed and applied for 133R. therryanumisolates fromPicea glehnii,P. jezoensisandAbies sachalinensisin a nursery and two planted sites in forests (forest sites) situating 10–20 km apart. Little genetic differentiation between the two forest sites suggests that gene flow had occurred between the forest sites. The nursery population was characterized by low genetic diversity compared to the two forest sites. Discriminant analysis of principal components (DAPC) indicated three genetically differentiated clusters ofR. therryanumwhich occurred sympatrically in each site. No dominant clusters were observed in the three host species, and no genetic differentiation was observed among the host species. The observed not significant linkage disequilibrium suggested the genetic recombination ofR. therryanum. Microsatellite markers developed in this study can be useful for the further population studies ofR. therryanum.
Published Version
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