Abstract
The aim of this study is to create and validate a fast, easy-to-use, affordable, sensitive, and accurate method for measuring Methotrexate and Folic acid in bulk and pharmaceutical products using Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC). A inerstsil ODS column was used with a running phase composed of 0.1% formic acid: methanol (60:40 v/v) at a flow rate of 1.0 ml/min. UV detection was used at a wavelength of 270 nm. Folic acid and Methotrexate correlation coefficients were found to be 0.999 over a concentration range of 2.5-15 µg/ml and 18.75-112.5 µg/ml, respectively. Methotrexate and Folic acid have respective retention times of 2.709 and 7.337 minutes. The run time for separating Folic acid and Methotrexate peaks was 10 minutes. This method, proposed as a regular analysis and quality control tool for medications that contain these active drugs either individually or in combinatio, was evident to be a suitable one. Keywords: HPLC, Folic acid, Methotrexate, Development, Validation
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
