Abstract
Due to the ease of genetic manipulation, yeast S. cerevisiae represent an attractive and accessible epigenetic model. However, isolation of proteins and protein complexes from yeast cells has always been a challenging and time‐consuming task associated with low yield and extensive degradation. To expand the utility of yeast as a powerful epigenetic tool, we developed a novel protocol for native chromatin purification. The protocol addresses numerous known, and previously unreported problems. Our method allows isolation of native nucleosomes with preserved combinatorial post translational modifications in a time‐ and cost‐effective manner. This procedure is suitable for downstream LC/MS analysis as well as recently developed matrix assisted reader chromatin capture (MARCC) and native chromatin precipitation (N‐chip) investigations.
Published Version
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