Abstract
Extraction of pure genomic DNA from microbes isolated from river sediments often hindered byco-isolated carryovers from the reagents used which reduces the yield and quality of DNA.Existing methods were lengthy, expensive and does not result in high yield of genomic DNA inshort time. This study describes a rapid and inexpensive DNA extraction method that involvedminimal purification step without phenol or other carcinogenic reagents. The microbial DNAextraction steps also does not require the use of special laboratory equipment. This method usesCetyltrimethylammonium bromide (CTAB) followed by ethanol based purification steps andwithin one hour the extraction of microbial DNA could be completed. The extracted genomicDNA from microbes isolated from river sediment of Sungai Kelantan, Malaysia using the methoddescribed in this study is of quality suitable for other downstream researches such asmetagenomics sequencing.
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