Abstract

The main objectives of this study were to evaluate the chemical constitution and allergenic potential of red propolis extract (RPE). They were evaluated, using high performance liquid chromatography (HPLC) and the release of β-hexosaminidase, respectively. A plethora of biologically active polyphenols and the absence of allergic responses were evinced. RPE inhibited the release of β-hexosaminidase, suggesting that the extract does not stimulate allergic responses. Additionally, the physicochemical properties and antibacterial activity of hydrogel membranes loaded with RPE were analyzed. Bio-polymeric hydrogel membranes (M) were obtained using 5% carboxymethylcellulose (M1 and M2), 1.0% of citric acid (M3) and 10% RPE (for all). Their characterization was performed using thermal analysis, Fourier transform infrared (FTIR), total phenolic content, phenol release test and, antioxidant activity through 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and Ferric Reducing Antioxidant Power (FRAP). The latter appointed to the similar antioxidant capacity of the M1, M2 and M3. The degradation profiles showed higher thermostability to M3, followed by M2 and M1. The incorporation of RPE into the matrices and the crosslinking of M3 were evinced by FTIR. There were differences in the release of phenolic compounds, with a higher release related to M1 and lower in the strongly crosslinked M3. The degradation profiles showed higher thermostability to M3, followed by M2 and M1. The antibacterial activity of the membranes was determined using the disc diffusion assay, in comparison with controls, obtained in the same way, without RPE. The membranes elicited antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis, with superior performance over M3. The hydrogel membranes loaded with RPE promote a physical barrier against bacterial skin infections and may be applied in the wound healing process.

Highlights

  • Hydrogels consist of a polymeric three-dimensional chain with considerable water and biological fluid absorption capacity

  • The red propolis extract (RPE)-loaded membrane is very promising, once RPE is a rich source of phenolic compounds, containing a huge variety of flavonoids

  • The citric acid used in membrane formulations as a crosslinking agent influenced the thermal stability and the release profile of phenolic compounds, revealing greater stability for M3 and greater release of these compounds by M1

Read more

Summary

Introduction

Hydrogels consist of a polymeric three-dimensional chain with considerable water and biological fluid absorption capacity. They are used as dressings for wounds and burns, due to their porous and hydrophilic characteristics. Polymeric matrices based on sodium carboxymethylcellulose (NaCMC) form transparent biofilms with high mechanical resistance and, healing potential when in contact with the skin. Natural products, used in traditional medicine, are among the options for incorporation in polymeric matrices, providing the synergistic effect to accelerate the wound healing process In this scenario, red propolis appears as an alternative for incorporation in polymeric matrices, due to its biological properties, cost-effectiveness and low adverse effect [3]

Objectives
Methods
Results
Discussion
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call