New Homogeneous Reverse Transcriptase and Nuclease Assays Based on Rare Earth Cryptate and Fluorescent Energy Transfer

Abstract

An homogeneous non isotopic technique based on time-resolved fluorescence, europium trisbipyridine cryptate (TBPEu3+) as fluorescent donor and non radiative energy transfer to an acceptor (cross-linked allophycocyanine, XL665) has been used to develop new assays. These formats provide rapid and straightforward measurement of reverse transcriptase (RTase) and DNase activity and for example a mean to screen for RTase inhibitors.