New Genome Sequence of an Echinaceapurpurea Endophyte, Arthrobacter sp. Strain EpSL27, Able To Inhibit Human-Opportunistic Pathogens.

Elisangela Miceli,Marco Fondi,Patrizia Bogani,Carolina Chiellini,Renato Fani,Gian Maria Rossolini,Luana Presta,Camilla Fagorzi,Valentina Maggini,Elena Perrin,Emanuele Bosi,Alessio Mengoni,Fabio Firenzuoli,Vincenzo Di Pilato

doi:10.1128/genomea.00565-17

Abstract

ABSTRACTWe announce here the draft genome sequence of Arthrobacter sp. strain EpSL27, isolated from the stem and leaves of the medicinal plant Echinacea purpurea and able to inhibit human-pathogenic bacterial strains. The genome sequencing of this strain may lead to the identification of genes involved in the production of antimicrobial molecules.

Highlights

We announce here the draft genome sequence of Arthrobacter sp. strain EpSL27, isolated from the stem and leaves of the medicinal plant Echinacea purpurea and able to inhibit human-pathogenic bacterial strains
EpSL27 genomic DNA was extracted using the cetyltrimethylammonium bromide (CTAB) method (6), and its authenticity has been confirmed by 16S rRNA gene sequencing
Whole-genome shotgun sequencing was performed with a 2 ϫ 300-bp paired-end approach using the MiSeq sequencing system (Illumina, Inc., San Diego, CA)

Summary

Introduction

We announce here the draft genome sequence of Arthrobacter sp. strain EpSL27, isolated from the stem and leaves of the medicinal plant Echinacea purpurea and able to inhibit human-pathogenic bacterial strains. The intriguing information obtained by the above-cited analyses led to whole sequencing of the strain genome. EpSL27 genomic DNA was extracted using the cetyltrimethylammonium bromide (CTAB) method (6), and its authenticity has been confirmed by 16S rRNA gene sequencing.

Results

Conclusion