Abstract
In addition to the extensive improvement programmes within fruit orchards and forest stands, considerable momentum is being generated in the application of genetic manipulation strategies to a variety of woody species. Several transformation approaches have been adopted for the production of transgenic trees. These include Agrobacterium-mediated gene delivery, chemical and/or electrical stimulated uptake of DNA into protoplasts, and the use of the newer technology of high velocity bombardment of plant tissues with DNA-coated particles. Transformation offers advantages over other genetic manipulation techniques, such as somatic hybridization, in that it allows the directed improvement of trees with minimum disruption of the genetic integrity of an elite genome. The information presented here is a comprehensive review of transformation in woody plants. Many of the advances have been made in this area during the last five years.
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