Abstract
Hybrid scaffolds based on bioactive glass (BAG) particles (<38 µm), covalently linked to gelatin (G*) using 3-glycidoxypropyltrimethoxysilane (GPTMS), have been studied for bone bioengineering. In this study, two glass compositions (13-93 and 13-93B20 (where 20% of the SiO2 was replaced with B2O3)) were introduced in the gelatin matrix. The Cfactor (gelatin/GPTMS molar ratio) was kept constant at 500. The hybrids obtained were found to be stable at 37 °C in solution, the condition in which pure gelatin is liquid. All hybrids were characterized by in vitro dissolution in Tris(hydroxymethyl)aminomethane (TRIS) solution (for up to 4 weeks) and Simulated Body Fluid (SBF) (for up to 2 weeks). Samples processed with 13-93B20 exhibited faster initial dissolution and significantly faster precipitation of a hydroxyapatite (HA) layer. The faster ion release and HA precipitation recorded from the G*/13-93B20 samples are attributable to the higher reactivity of borosilicate compared to silicate glass. The MC3T3-E1 cell behavior in direct contact with the hybrids was investigated, showing that the cells were able to proliferate and spread on the developed biomaterials. Tailoring the glass composition allows us to better control the material’s dissolution, biodegradability, and bioactivity. Bioactive (especially with 13-93B20 BAG) and biocompatible, the hybrids are promising for bone application.
Highlights
IntroductionPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations
We present hybrid materials for bone tissue engineering based on gelatin and bioactive glass (BAG) (Figure 1), where two BAGs, with different compositions (13-93 and 13-93B20), are compared. 13-93 is an Food and Drug Administration (FDA)-approved BAG that exhibits a slower dissolution rate than commercialized BAGs 45S5 or S53P4 [21,22]
The aim of this study is to develop hybrid materials based on gelatin and BAG able to release therapeutic ions for bone regeneration while controlling the dissolution properties of the organic and inorganic phases of the scaffold
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. For a large loss of bone substance (defect greater than 1 cm3 ) following a traumatic situation as a pathology or accidental defect, the natural process of self-repair is compromised [1]. Tissue engineering is an innovative approach used for bone repair. Bone reconstruction is assisted with materials that participate in tissue regeneration [2,3]. These materials must have properties adapted to this function
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call