New experimental technique to study blood cardioplegia in the isolated, perfused rat heart

Abstract

Blood catdioplegia has been extensively studied clinically and in the large animal experimental model. We describe here a modification of the original Langendorff technique to study continuous warm blood cardioplegia in the isolated, perfused rat heart. The excised heart is mounted on the perfusion apparatus and perfused with Krebs-Henseleit buffer. Prearrest hemodynamics are recorded. The shed blood in the mediastinal cavity (8 to 12 mL) is collected, filtered, and reconstituted into cardioplegic solution (hematocrit, 0.20; K +, 15 mmol/L). Hearts are arrested and maintained at 37 °C by continuous recirculation of blood cardioplegia for 1 hour. The blood cardioplegia system consists of a Silastic tubing oxygenator, peristaltic pump, and two filters (40 μm pore size). The heart is reperfused with Krebs-Henseleit solution, and postarrest hemodynamics are recorded. Percentage recovery of peak left ventricular pressure, heart rate, and coronary flow were 98.5 ± 3.1, 102 ± 4.2, and 98.5 ± 4.5 (mean ± standard error of the mean; n = 6), respectively. Myocardial oxygen consumption during arrest was 57 μL · min −1 · g −1 dry wt, which is 10% of the myocardial oxygen consumption of a beating heart in in-vivo and ex-vivo models. These results suggest the feasibility of studying blood cardioplegia in the isolated, perfused rat heart model under controlled conditions. Continuous warm blcod cardioplegic arrest provided excellent myocardial protection for 1 hour in this model.