Abstract
New Delhi Metallo-β-Lactamase, Ontario, Canada
Highlights
To the Editor: The New Delhi metallo-β-lactamase (NDM-1) was first characterized in 2009 from Klebsiella pneumoniae and Escherichia coli isolated from a patient in Sweden who had received medical care in New Delhi, India [1]
K. pneumoniae GN529 was highly resistant to all β-lactams, aminoglycosides, quinolones, tetracycline, nitrofurantoin, and co-trimoxazole
A transconjugant E. coli positive for blaNDM-1 (E. coli J529, Table) was resistant to all β-lactams and aminoglycosides tested
Summary
To the Editor: The New Delhi metallo-β-lactamase (NDM-1) was first characterized in 2009 from Klebsiella pneumoniae and Escherichia coli isolated from a patient in Sweden who had received medical care in New Delhi, India [1]. Primers were designed (NDMF, 5′-AATGGAATTGCCCAATATT ATGC-3′; NDM-R, 5′-CGAAAGTCA GGCTGTGTTG C-3′) for the specific detection of blaNDM-1 and included in 1 of the multiplex PCRs (multiplex V). Primers NDM-F and NDM-R2 (5′-TCAGCGCAGCTTGTCGGC-3′) were used to amplify and sequence the entire blaNDM-1 gene. Testing colistin breakpoint for Enterobacteriaceae: susceptibility
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