New biochemical methods for production of Nα-benzyloxycarbonyl- d, l-aminoadipic-δ-semialdehyde and Nα-benzyloxycarbonyl- d, l-aminoadipic acid

Abstract

New biochemical methods for the production of N α-benzyloxycarbonyl- l-aminoadipate δ-semialdehyde ( N α- Z- l-AASA), N α- Z- d-AASA, N α- Z- l-aminoadipic acid ( N α- Z- l-AAA), N α- Z- d-AAA were developed using cells of Rhodococcus sp. AIU Z-35-1. When the cells harvested after 1 day of cultivation were incubated with 100 mM N α- Z- l-lysine at pH 5.0 for 1 day at 30 °C or at pH 7.0 for 2 days at 10 °C, more than 95 mM N α- Z- l-AASA was produced. Using the cells harvested after 3 days of cultivation, N α- Z- l-AAA was efficiently produced by incubating at pH 7.0 for 4 days at 30 °C. Similar conversion yields of N α- Z- d-AASA and N α- Z- d-AAA were also obtained under the same conditions. Thus, the microbial methods proposed here were superior to the chemical and other biochemical methods in simplicity, reaction rate, and yield.