New approaches in identifying of diatom plankton in biological fluids and water

Abstract

The aim of the research was to introduce in practice the most optimal method for detecting diatom plankton in cases of death from drowning in water.Materials and methods. The research received biological objects from 32 corpses that died as a result of drowning in water, namely, the fluid from the sinus of sphenoid bone (19 glases) and pericardial fluid (32 glases). Next step was staining of microspeciments by Romanovsky-Giemsa method and their microscopy.Results. The use of a mixture of Romanovsky-Giemsa paints while coloring the structures of diatoms can reveal the features of the structure of diatoms, describe them and refer them to a certain family and class, which makes the research more meaningful.Conclusions. Detection of diatom plankton in a liquid from the sinus of sphenoid bone and in the pericardial fluid has significant advantages over its detection in mineralizat from the tissue of the kidney (lungs, liver and bone marrow). The method of staining agents used promotes the rapid and qualitative verification of plankton, its quantitative and qualitative composition, which allows us to express an assumption about the place of death from mechanical asphyxiation by drowning in water.