Abstract
Despite the rapid advances in chromatographic technology, the development of high-performance liquid chromatographic methods remains expensive with respect to both time and materials. A new approach is proposed here which is suitable for the investigation of eluents and packings for the resolution of multicomponent samples. This is based upon the use of a small column to mimic the retentive characteristics of isocratic analytical systems but on a greatly reduced time scale. The general utility of this approach is illustrated by three applications, using data for purines and pyrimidines on both reversed-phase an ion-exchange systems.
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