Abstract
Developing chromatographic methods can be laborious, time-consuming, and expensive. The definition of a strategy to find rapid separation conditions has therefore become of prime importance since the analytical development has to follow the rhythm of the generation of new compounds. To meet this demand, chemists clearly need the utilization of automation and standardized strategies of work. This paper reports a standardized and fully automated approach dedicated to the development of liquid chromatographic (LC) methods, which are widely used to provide information about chemical reactions, such as impurity profiles and structure or potency determinations. The present approach consists of automatically screening samples with predefined chromatographic conditions. Chromatography is performed under the gradient mode, and the whole dataset is generated in less than 1 day. Even if the screening does not directly provide the final conditions for the separation of the compounds of interest, it generally gives sufficient information to allow a rapid optimization of the chromatographic separation (typically less than 24 h). Different examples of development of LC methods using this strategy are presented.
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