Abstract
Purpose: To isolate and evaluate α-glucosidase inhibitors from dichloromethane extract of Croton bonplandianum Baill as probable remedy for management of diabetes. Methods: Activity-guided isolation of constituents from dichloromethane extract was carried out. Fractionation of dichloromethane extract by column chromatography on silica gel and Sephadex LH 20 using different mobile phase systems led to the isolation of compounds (A-I). The structures of these isolated compounds were established by ultraviolet (UV) and infrared (IR) spectroscopy. Proton nuclear magnetic resonance ( 1 H NMR), 13 C NMR and mass spectrophotometry, electron impact mass spectroscopy (EIMS) and high resolution mass spectroscopy (HRMS) were used for structural elucidation. All the isolated compounds were screened for their α-glucosidase inhibitory activity using standard in vitro α-glucosidase inhibition assay. Acarbose was used as positive control. Results: On the basis of their physical and spectral data from literature, the isolated compounds were identified as n-pentacosanyl-n-nonadeca-7′-en-9′-α-ol 1′-oate (A), n-tridecanyl n-octadec-9,12-dienoate (B), nonacosyl hexadecanoate (C), heptacosanoic acid (D), 1,3,5-trihydroxy-2-hexadecanoylamino- (6e,9e) heptacosdiene (E),cumarin (F), betulin (G), stigmasterol (H), and 3,5-dimethoxy 4-hydroxy cinnamic acid (I).Compounds F, GandI possessed significant α glucosidase inhibitory activity in a concentration-dependent manner with 50 % inhibitory concentration (IC 50 ) values ranging from 23.0 to 26.7 μg/mL, relative to that of the positive control, acarbose (IC 50 , 38.2 μg/mL). Conclusion: The plant contains bioactive compounds with α-glucosidase inhibitory activity. This lendssome support for the traditional use of this herb in the management of diabetes. Compound F, GandI possessed significant α glucosidase inhibitory activity in a concentration-dependent manner and may be developed as a new α-glucosidase inhibitor. Keywords: α-Glucosidase, Inhibition, Diabetes, Acarbose, Cumarin, Betulin, 3,5 Dimethoxy 4-hydroxy cinnamic acid, Croton bonplandianum
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
