Abstract
ObjectiveThe present study aimed to explore the clinical significance of neutrophils infiltration and carcinoembryonic antigen related cell adhesion molecule 1 (CEACAM1) expression in the tongue squamous cell carcinoma (TSCC), and to probe the possible relationship between them.Materials and MethodsTissue microarray and immunohistochemistry were used to detect neutrophils density and CEACAM1 expression in 74 cases of primary TSCC specimens and 17 cases of corresponding peritumoral tissues. The relationship of CEACAM1 expression and neutrophils density with clinicopathologic parameters and cancer-related survival of TSCC patients were evaluated. The correlation between CEACAM1 expression and neutrophils density was also evaluated. Real-time quantitative transcription polymerase chain reaction (qRT-PCR) was used to explore the possible molecular mechanisms between CEACAM1 expression and neutrophils infiltration.ResultsImmunohistochemistry evaluation revealed that there was more neutrophils infiltration in TSCC tissues than in peritumoral tissues. High neutrophil density was associated with LN metastasis (P = 0.01), higher clinical stage (P = 0.037) and tumor recurrence (P = 0.024). CEACAM1 overexpression was also associated with lymph node metastasis (P = 0.000) and higher clinical stage (P = 0.001). Survival analysis revealed that both neutrophils infiltration and CEACAM1 overexpression were associated with poorer cancer-related survival of TSCC patients (P<0.05), and neutrophils infiltration was an independent prognostic factor for TSCC (P<0.05). Furthermore, overexpression of CEACAM1 was correlated with more neutrophils infiltration in TSCC tissues (P<0.01). qRT-PCR results showed that CEACAM1-4L can upregulate the mRNA expression of IL-8 and CXCL-6, which were strong chemotactic factors of neutrophils.ConclusionOur results demonstrated that more neutrophils infiltration and overexpression of CEACAM1 were associated with poor clinical outcomes in TSCC tissues. Overexpression of CEACAM1 on tumor cells correlated with more neutrophils infiltration to some extent through upregulating mRNA expression of IL-8 and CXCL-6.
Highlights
Oral squamous cell carcinoma (OSCC) is among the ten most frequently diagnosed cancers in the world, while the tongue squamous cells carcinoma (TSCC) is the most common type in OSCC [1,2]
Survival analysis revealed that both neutrophils infiltration and carcinoembryonic antigen related cell adhesion molecule 1 (CEACAM1) overexpression were associated with poorer cancer-related survival of TSCC patients (P,0.05), and neutrophils infiltration was an independent prognostic factor for TSCC (P,0.05)
Overexpression of CEACAM1 was correlated with more neutrophils infiltration in TSCC tissues (P,0.01). quantitative transcription polymerase chain reaction (qRT-PCR) results showed that CEACAM1-4L can upregulate the mRNA expression of IL-8 and CXCL-6, which were strong chemotactic factors of neutrophils
Summary
Oral squamous cell carcinoma (OSCC) is among the ten most frequently diagnosed cancers in the world, while the tongue squamous cells carcinoma (TSCC) is the most common type in OSCC [1,2]. Inflammatory cells include neutrophils, lymphocytes, plasmocytes, macrophages, esinophilic granulocytes etc. TANs have been reported to be closely associated with tumor progression and tumor vasculature [7]. The presence of intratumoral neutrophils is a poor prognostic factor in several types of tumors, including localized renal cell carcinoma [8], gastric adenocarcinoma [9], intrahepatic cholangiocarcinoma [10], and hepatocellular carcinoma [11] etc. The study from Trellakis S et al showed that human head and neck cancer tissues exhibited considerable infiltration by polymorphonuclears (PMNs), and strong infiltration was associated with poorer survival in advanced disease [12]. The research of neutrophils infiltration in TSCC tissues has rarely been reported. How to regulate the inflammatory status of cancers seems important
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