Abstract
This study describes an in vitro investigation on the role of Pasteurella multocida cells and its isolated protein Hand LPS on neutrophil adhesion and migration through bovine endothelial cell monolayers. Pmulticoda cells, protein Hand LPS increased the adhesion and transmigration of neutrophils through BAECThe bacteria/cell ratio of 100 for Pmultocida, protein H concentration 0.05–0.2 μM and LPS concentration 0.5–1.0 μLM respectively, induced the maximum adhesion and transmigration of neutrophils through BAECThe optimal time of incubation with bacteria or bacterial products was 4–6 hOur results confirm the role of Gram-negative bacteria and of components of the outer membrane such as protein H or LPS in activating the neutrophils and in promoting the adhesion and cells transmigration from the vessels to the site of inflammation.
Published Version
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