Neuroprotective action of aspirin on Paraquat intoxication in on Drosophila melanogaster

Mayslla Keylla Brito Do Carmo,Anderson Oliveira Souza,Carlos Antonio Couto Lima,Joyce Marinho De Souza,Mayara Oliveira Vidotto Figueiredo

doi:10.33448/rsd-v10i4.14179

Abstract

Acetylsalicylic acid or aspirin is the most widely used drug globally for its anti-inflammatory characteristics, although little is known about its actions on the central nervous system (CNS). We investigated aspirin's potential neuroprotective effects against paraquat-induced neurotoxicity (PQ) in the present study. Adult male wild-type flies were exposed to a diet containing PQ (3 mM) and/or aspirin (1 μM; 5 μM; 10 μM). Flies fed with PQ reduced locomotion and increased mortality. PQ-induced neurotoxicity has also been associated with a marked decrease in acetylcholinesterase (AChE) activity and lipid peroxidation. Co-exposure to aspirin (5 μM) increased survival, improved motor performance, increased AChE activity, and decreased lipid peroxidation. Our results suggest aspirin's neuroprotective effects, probably due to its lysosomal action and antioxidant characteristics. Thus, we demonstrate that the Drosophila melanogaster model can elucidate basic aspirin mechanisms to assist the evaluations carried out in higher animals to minimize the neurodegenerative effects caused by diseases such as Parkinson's and Alzheimer's.

Highlights

Neurodegenerative diseases are a heterogeneous group of disorders characterized by progressive degeneration of the central nervous system (CNS) structure and function or peripheral nervous system
Males and females were kept in bottles containing 25 mL of traditional medium (Diet) and synchronized so that Fl could be collected after they emerge from the pupal
We observed that the PQ concentration of 1 and 2 mM showed almost no difference compared to the control group, except on the last day, where increased the death rate

Summary

Introduction

Neurodegenerative diseases are a heterogeneous group of disorders characterized by progressive degeneration of the CNS structure and function or peripheral nervous system. The most common neurodegenerative diseases include Alzheimer's disease and Parkinson's disease (Erkkinen et al, 2018). Together, these diseases affect more than 50 million people worldwide (Livingston et al, 2020). Synaptic connection interruption resulting in cerebral atrophy, causing the symptoms most observed in AD patients, such as cognitive skills deficit and memory loss (Iqbal et al, 2010). Parkinson's disease, on the other hand, is characterized by motor imbalances with the loss of dopaminergic neurons in the substantia nigra in the brain and the appearance of Lewy bodies, cytoplasmic inclusions formed by alpha-synuclein (Baltazar et al, 2014)

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