Neuroneuronal interconnections in the rat superior cervical ganglion; Possible anatomical bases for modulatory interactions revealed by intracellular horseradish peroxidase labelling

Abstract

Electrophysiologically identified neurons of rat superior cervical ganglion were intracellularly injected with horseradish peroxidase and processed for light and electron microscopic observation. At light microscope level, neurons could be classified according to their dendritic arborization pattern in the vicinity of the soma into radiate, tufted and intermediate types. Upon electrical stimulation of the internal and external carotid nerves it was observed that radiate and intermediate neurons sent their axons into one or the other of these nerve trunks, whereas a majority of tufted neurons gave no response to stimulation of either of these postganglionic nerves. Electron microscopic exploration of horseradish peroxidase-labelled neurons revealed a surprisingly high prevalence of interconnectivity between ganglionic neurons. These contacts were both dendrosomatic and dendrodendritic, and were a universal feature of the labelled neurons explored. Twenty-two of the 23 labelled cells were found to receive direct dendritic appositions on their somata, and 13 of these 23 cells were seen each to send their dendrites into contact with at least one unlabelled neuronal soma. Dendrodendritic contacts were observed for 87% of the labelled neurons, and most of the cells (80%) were seen to form triadic contacts which included two dendrites and a preganglionic nerve ending. All these figures represent minimum incidences. None of the dendrosomatic or dendrodendritic appositions observed was overtly synaptic although several morphological features indicated the possibility of somatic and or dendritic release and uptake at sites of apposition. It is suggested that the observed appositions provide anatomical substrates for modulatory interactions between the ganglionic neurons, possibly involving slow potentials or the switching of metabolic pathways.