Neuronal excitability is dose-dependently lessened by plant lignan arctigenin via inhibition of kainate receptors

Abstract

Event Abstract Back to Event Neuronal excitability is dose-dependently lessened by plant lignan arctigenin via inhibition of kainate receptors Sándor Borbély1*, Attila Tóth1, Gergely Jócsák1, Elek Molnár2, László Détári1 and Ildikó Világi1 1 Eötvös University, Department of Physiology and Neurobiology, Hungary 2 University of Bristol, Medical Research Council Centre for Synaptic Plasticity, Department of Anatomy, United Kingdom Plant lignans, important biologically active compounds, exert considerable effects on mammalian tissues. Some lignans, like arctigenin posses antitumor, anti inflammatory, antiviral and antioxidant properties. Furthermore, it was also reported that arctigenin – a member of phenolic molecule family – has neuroprotective activity against glutamate-induced toxicity in primary neuronal cultures and its direct interaction with kainate receptors (KARs) [Jang et al. (2002) J Neurosci Res 68:233-40]. In the present study the effect of arctigenin, produced by plant tissue cultures or obtained commercially was tested. In vitro electrophysiological experiments were performed on cortical slices of adult rats, and changes of evoked field potentials were tested in somatosensory cortex. Alterations of the early and late components of evoked responses were evaluated. Furthermore, the effect of arctigenin on evoked field potentials was investigated in in vivo electrophysiological experiments, using anesthetized rats. [3H]kainate ligand binding studies of recombinant KARs expressed in HEK-293 cells were performed to establish the subunit selectivity of arctigenin/KAR interaction. Co2+-uptake assay was used to assess arctigenin-induced changes in non NMDA receptors mediated Ca2+ influx into neurones. Our results indicate that arctigenin dose dependently inhibited both components of the evoked response in brain slices and the same effect was observed in layer 3 evoked responses recorded from anesthetized rats. According to receptor binding studies antagonistic effect of arctigenin is partly mediated by the inhibition of KARs. Ca2+ permeability of non NMDA receptors was also dose dependently reduced by arctigenin in somatosensory cortex. Our results demonstrated the antagonistic effect of arctigenin on non NMDA receptors in rat somatosensory cortex. Since non NMDA receptors are involved in different brain diseases, a low-affinity antagonist compound can be useful in medical treatments. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Disorders of the nervous system Citation: Borbély S, Tóth A, Jócsák G, Molnár E, Détári L and Világi I (2010). Neuronal excitability is dose-dependently lessened by plant lignan arctigenin via inhibition of kainate receptors. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00029 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 19 Apr 2010; Published Online: 19 Apr 2010. * Correspondence: Sándor Borbély, Eötvös University, Department of Physiology and Neurobiology, Budapest, Hungary, bosa@ludens.elte.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sándor Borbély Attila Tóth Gergely Jócsák Elek Molnár László Détári Ildikó Világi Google Sándor Borbély Attila Tóth Gergely Jócsák Elek Molnár László Détári Ildikó Világi Google Scholar Sándor Borbély Attila Tóth Gergely Jócsák Elek Molnár László Détári Ildikó Világi PubMed Sándor Borbély Attila Tóth Gergely Jócsák Elek Molnár László Détári Ildikó Világi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.