Modulation of kainate receptor activity in isolated brain slices

Abstract

Event Abstract Back to Event Modulation of kainate receptor activity in isolated brain slices Ildikó Világi1* 1 Eötvös Loránd University, Department of Physiology and Neurobiology, Hungary Ionotropic glutamate receptors are responsible for the fast synaptic activation in the central nervous system. Kainate types of them (GluK1-5) are widely expressed in the central nervous system, however, the AMPA- or NMDA type receptors are better characterized because of long-time lack of pharmacological specificity. Kainate receptors play an important role in regulation complex network activities. Presynaptically they contribute to the control of synaptic transmitter release, while postsynaptically they possess neuronal excitability regulatory function. Kainate receptors play important role in physiological functions like memory tasks, but they are also referred in connection with some pathological processes resembling epilepsy or neuronal excitotoxicity. Recently some specific kainate receptor antagonists were introduced, for instance ACEA or LY466195. A plant lignan, arctigenin was also reported to have specific kainate receptor antagonistic effect (Jang et al. (2002) J Neurosci Res 68:233-40). Arctigenin exerted neuroprotective effect against glutamate-induced toxicity in primary cultured rat cortical cells. In an isolated brain slice study the inhibitory effect of arctigenin was investigated. Experiments were performed on rat somatosensory cortex and hippocampus, and changes of field responses evoked by electrical stimulation were analyzed. Parallel kainate induced Co2+-uptake assay was carried out to test the alteration in ion-passing. According to the results arctigenin effectively and dose-dependently lessened the evoked responses in both brain regions. It also decreased the amount of Co2+, which could cross the membrane through activated non-NMDA receptors in the investigated area. It was also proved that arctigenin can cross the blood-brain barrier and can bind to GluK1 receptor. So this natural compound offers a good opportunity for study specific kainate receptor dependent physiological and pathological processes. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Oral Presentation Topic: Kainate receptors in synaptic plasticity Citation: Világi I (2010). Modulation of kainate receptor activity in isolated brain slices. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00207 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 May 2010; Published Online: 04 May 2010. * Correspondence: Ildikó Világi, Eötvös Loránd University, Department of Physiology and Neurobiology, Budapest, Hungary, vilagildi@ludens.elte.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ildikó Világi Google Ildikó Világi Google Scholar Ildikó Világi PubMed Ildikó Világi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.