Neurochemical investigations on parathyroid hormone 2 receptor-immunoreactive neurons in the human and macaque hypothalamus and brainstem

Abstract

Event Abstract Back to Event Neurochemical investigations on parathyroid hormone 2 receptor-immunoreactive neurons in the human and macaque hypothalamus and brainstem Attila G. Bagó1, 2*, Dimitrov Eugene3, Richard Saunders4, Laszlo Seress5, Miklos Palkovits1, 2, Ted B. Usdin3 and Arpad Dobolyi1, 2 1 HAS - Semmelweis University, Neuromorphological and Neuroendocrine Research Laboratory, Department Anatomy, Histology and Embryology, Hungary 2 National Institute of Neurosurgery, Hungary 3 National Institute of Mental Health, Section on Fundamental Neuroscience, United States 4 National Institute of Mental Health, Laboratory of Neuropsychology, United States 5 University of Pécs, Central Electron Microscopic Laboratory, Hungary Parathyroid hormone receptor 2 (PTH2R) and its ligand, tuberoinfundibular peptide of 39 residues (TIP39) constitute a neuromodulator system implicated in endocrine and nociceptive regulations. We described the presence and distribution of the PTH2R and TIP39 in macaque and human brain. In situ hybridization histochemistry showed TIP39 mRNA only in the thalamic subparafascicular area and the pontine medial paralemniscal nucleus. In contrast, high levels of PTH2R expression were identified in a variety of hypothalamic and brainstem areas in macaque, which correlated well with RT-PCR data in human. Immunohistochemically, a widespread distribution of PTH2R-immunoreactive fibers was described in human, including dense fiber networks in the medial preoptic area, hypothalamic paraventricular, periventricular and infundibular (arcuate) nuclei, lateral hypothalamic area, median eminence, periaqueductal gray, lateral parabrachial nucleus, nucleus of the solitary tract, sensory trigeminal, and medullary dorsal reticular nuclei. Co-localization of PTH2R immunoreactivity with vesicular glutamate transporter 2 suggested that PTH2R terminals are glutamatergic. In addition, PTH2R co-localized with somatostatin in neuronal cell bodies in the hypothalamic periventricular nucleus as well as in fibers located in the median eminence suggesting that TIP39 may directly influence hypophysiotropic somatostatin-containing neurons. In contrast, numerous PTH2R-containing terminals closely appose corticotropin releasing-hormone containing neurons suggesting a presynaptic action of TIP39 on terminals innervating these cells. The results support that TIP39 and the PTH2R are expressed in the brain of primates and this peptide neuromodulator system is able to influence target neurons with different mechanisms. The work was supported by the Bolyai Fellowship of the HAS, NKTH-OTKA K67646 and NFM-OTKA NNF78219 for AD, OTKA NK72929 for MP, and NIMH Intramural Research Program for TBU. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Homeostatic and neuroendocrine systems Citation: Bagó AG, Eugene D, Saunders R, Seress L, Palkovits M, Usdin TB and Dobolyi A (2010). Neurochemical investigations on parathyroid hormone 2 receptor-immunoreactive neurons in the human and macaque hypothalamus and brainstem. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00076 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Apr 2010; Published Online: 21 Apr 2010. * Correspondence: Attila G Bagó, HAS - Semmelweis University, Neuromorphological and Neuroendocrine Research Laboratory, Department Anatomy, Histology and Embryology, Budapest, Hungary, bagoatt@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Attila G Bagó Dimitrov Eugene Richard Saunders Laszlo Seress Miklos Palkovits Ted B Usdin Arpad Dobolyi Google Attila G Bagó Dimitrov Eugene Richard Saunders Laszlo Seress Miklos Palkovits Ted B Usdin Arpad Dobolyi Google Scholar Attila G Bagó Dimitrov Eugene Richard Saunders Laszlo Seress Miklos Palkovits Ted B Usdin Arpad Dobolyi PubMed Attila G Bagó Dimitrov Eugene Richard Saunders Laszlo Seress Miklos Palkovits Ted B Usdin Arpad Dobolyi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.