Neurochemical and electrophysiological investigation of amygdala and olfactory cortex interplay during odor fear conditioning

Abstract

Event Abstract Back to Event Neurochemical and electrophysiological investigation of amygdala and olfactory cortex interplay during odor fear conditioning Sandrine Parrot1*, Chloe Hegoburu2, Yannick Sevelinges2, Remi Gervais2 and Anne-Marie Mouly2 1 University of Lyon, NeuroChem Department, France 2 CNRS-University of Lyon, NSCC laboratory, France Fear conditioning paradigm has been widely used for studying the neurobiology of learning and memory. It consists of pairing an initially neutral stimulus (CS) with an aversive unconditioned stimulus (US). Subsequent re-exposure to the CS induces a learned fear response. We previously showed that olfactory fear conditioning induces synaptic changes in the basolateral amygdala (BLA) and posterior piriform cortex (PPC) suggesting an involvement of these structures in the learning. The present work was aimed at further characterizing the learning-induced modifications in these two sites. We first used high temporal resolution microdialysis(1-min sampling rate) on freely-moving rats to investigate the variations of neurotransmitter concentrations in BLA and PPC during odor fear acquisition. Simultaneous monitoring of GABA and Glutamate was performed in both PPC and BLA during the conditioning session including six odor(CS)-shock(US) pairings. We observed a temporal dynamic of activation of these structures during the course of successive pairings, with an early response of the BLA during the first CS-US association preceding neurotransmitter release in CPP after which CPP alone shows training-related modifications. These data suggest that the projection pathway between BLA and CPP could play a crucial role in sustaining the observed dynamic. Further experiments are therefore carried out to study this pathway. In a current study, we are recording the field potential signals induced in the PPC in response to electrical stimulation of the BLA, and investigating whether this pathway can be experimentally potentiated. In parallel, we are studying the consequences of an endogenous increase of glutamate in the BLA on the variations of glutamate and GABA in the CPP. These experiments should increase our understanding of the interactions between cortical and limbic structures during odor fear conditioning. Conference: 41st European Brain and Behaviour Society Meeting, Rhodes Island, Greece, 13 Sep - 18 Sep, 2009. Presentation Type: Poster Presentation Topic: Poster presentations Citation: Parrot S, Hegoburu C, Sevelinges Y, Gervais R and Mouly A (2009). Neurochemical and electrophysiological investigation of amygdala and olfactory cortex interplay during odor fear conditioning. Conference Abstract: 41st European Brain and Behaviour Society Meeting. doi: 10.3389/conf.neuro.08.2009.09.253 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Jun 2009; Published Online: 12 Jun 2009. * Correspondence: Sandrine Parrot, University of Lyon, NeuroChem Department, Lyon, France, sparrot@sante.univ-lyon1.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sandrine Parrot Chloe Hegoburu Yannick Sevelinges Remi Gervais Anne-Marie Mouly Google Sandrine Parrot Chloe Hegoburu Yannick Sevelinges Remi Gervais Anne-Marie Mouly Google Scholar Sandrine Parrot Chloe Hegoburu Yannick Sevelinges Remi Gervais Anne-Marie Mouly PubMed Sandrine Parrot Chloe Hegoburu Yannick Sevelinges Remi Gervais Anne-Marie Mouly Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.