Neural progenitor cell-derived nanovesicles promote hair follicle growth via miR-100

Lei Cao,Mifang Yang,Yuanbo Huang,Shiqin Tao,Xiaohong Zhu,Lei Wang,Jing Gu,Tian Tian,Wenrong Xu,Guangdong Feng,Rushan Xia,Yinni Ma

doi:10.1186/s12951-020-00757-5

Abstract

BackgroundAccumulating evidence shows that mesenchymal stem cell-derived extracellular vesicles (EVs) hold great promise to promote hair growth. However, large-scale production of EVs is still a challenge. Recently, exosome-mimetic nanovesicles (NV) prepared by extruding cells have emerged as an alternative strategy for clinical-scale production. Here, ReNcell VM (ReN) cells, a neural progenitor cell line was serially extruded to produce NV.ResultsReN-NV were found to promote dermal papilla cell (DPC) proliferation. In addition, in a mouse model of depilation-induced hair regeneration, ReN-NV were injected subcutaneously, resulting in an acceleration of hair follicle (HF) cycling transition at the site. The underlying mechanism was indicated to be the activation of Wnt/β-catenin signaling pathway. Furthermore, miR-100 was revealed to be abundant in ReN-NV and significantly up-regulated in DPCs receiving ReN-NV treatment. miR-100 inhibition verified its important role in ReN-NV-induced β-catenin signaling activation.ConclusionThese results provide an alternative agent to EVs and suggest a strategy for hair growth therapy.

Highlights

Accumulating evidence shows that mesenchymal stem cell-derived extracellular vesicles (EVs) hold great promise to promote hair growth
This study provides the potential for developing ReNcell VM (ReN)-NV as a novel strategy for promoting hair growth
We evaluated the amount of the miRNAs in dermal papilla cell (DPC) after incubation with PBS, 293-NV or ReNNV

Summary

Introduction

Accumulating evidence shows that mesenchymal stem cell-derived extracellular vesicles (EVs) hold great promise to promote hair growth. Large-scale production of EVs is still a challenge. Exosomemimetic nanovesicles (NV) prepared by extruding cells have emerged as an alternative strategy for clinical-scale production. Hair loss, characterized by shorter anagen and longer telogen phases of hair follicles (HF), is a common medical problem which may cause both cosmetic and psychological problems. Finasteride, platelet-rich plasma (PRP), low-level laser therapy (LLLT), stem cell therapy, and HF transplantation are current treatment approaches. HF, which contains both epithelial and mesenchymal compartment, is a complicated organ which undergoes cycles of growth (anagen), regression (catagen), quiescence (telogen) and regeneration [10].

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