Abstract
Anthocyanins, a group of flavonoids, are widely present in plants and determine the colors of the peels of stems, fruits, and flowers. In this study, we used UHPLC-ESI-MS to identify anthocyanins in the herbal plant Dendrobium officinale, which has been used for centuries in China. The results indicated that the total anthocyanin content in samples from Guangxi was the highest. Seven anthocyanins were identified, and the fragmentation pathways were proposed from D. officinale. Most of the identified anthocyanins were composed of cyanidin and sinapoyl groups. We also carried out that the sinapoyl group had active sites on breast cancer receptors by using Schrödinger. The relative levels of the 7 anthocyanins in the samples from the three locations were determined. Transcriptomic analysis was used to analyze the sinapoyl anthocyanin synthesis-related genes in plants, such as genes encoding UGTs and serine carboxypeptidase. We speculated that sinapoyl anthocyanin biosynthesis was associated with the activities of certain enzymes, including chalcone flavonone isomerase-like, hydroxycinnamoyltransferase 1, UGT-83A1, UGT-88B1 isoform X1, serine carboxypeptidase-like 18 isoform X3, and serine carboxypeptidase-like 18.
Highlights
Dendrobium officinale is a widely used herb in China and southern Asian countries
Peak 1 was identified as cyanidin 3-[2-(glucosyl)-6-(sinapoyl)glucoside]-5-glucoside [20, 21], C44H51O25+, which exhibited a retention time of 10.944 min by UV chromatography
The results showed that cyanidin 3-[2-(glucosyl)-6-(sinapoyl)glucoside]-5-glucoside was successfully docked into the breast cancerrelated proteins, CTSD and CA9
Summary
Dendrobium officinale is a widely used herb in China and southern Asian countries. This plant was first recorded as a traditional Chinese medicine in Shennong’s Classic of Materia Medica, which was written more than 2000 years ago [1, 2]. Most reports on anthocyanins have focused on different kinds of flowers and the peels or seeds of fruits, such as grape [8]. A previous report has stated that there exist substantial differences in anthocyanin content between D. officinale plants with green and red peels. The related reports of qualitative differences of D. officinale among different areas are rare Those genes involved in regulating the red peels are still defective
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
