Nephroprotective Activities of Ethanolic Roots Extract of <em>Pseudocedrela kotschyi</em> against Oxidative Stress and Nephrotoxicity in Alloxan-induced Diabetic Albino Rats

Abstract

Introduction: The present study was designed to evaluate the nephroprotective activities of ethanolic roots extract of Pseudocedrela kotschyi against oxidative stress and nephrotoxicity in alloxan induced diabetic albino rats. Methodology: Diabetes was induced in Albino rats by administration of alloxan monohydrate (150 mg/kg, i.p). The ethanolic roots extract of Pseudocedrela kotschyi at a dose of 250 and 500 mg/kg body weight was administered at single dose per day to diabetes induced rats for a period of 28 days. The effect of ethanolic roots extract of Pseudocedrela kotschyi on blood glucose, Urea, Creatinine, renal oxidative stress markers and lipid peroxidation were measured in the diabetic rats. Results: The ethanolic roots extract of Pseudocedrela kotschyi exhibited significant reduction of blood glucose (p<0.05) at the dose of 250 and 500 mg/kg when compared with the standard drug Glibenclamide (10 mg/kg). Urea and Creatinine levels were significantly increased (p<0.05) in diabetic group without treatment as compared to control. In addition, the level of oxidative stress markers such as Superoxide Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx), Glutathione (GSH) were significantly decreased (p<0.05) in diabetic rats as compared to normal rats while the lipid peroxidation (MDA) significantly increased (p<0.05) in diabetic group without treatment as compared to control (normal) rat. Apart from these, histopathological changes also revealed the cytoprotective nature of the ethanolic roots extract of Pseudocedrela kotschyi against alloxan induced necrotic damage of renal tissues. Conclusion: From the above results, we concluded that the ethanolic roots extract of Pseudocedrela kotschyi can prevent renal damage from alloxan induced nephrotoxicity in rats and it is likely to be mediated through its antioxidant activities.