Neopapillarine, an Unusual Coumarino-Alkaloid from the Root Extract of Neocryptodiscus papillaris with Cytotoxic Activity on Renal Cancer Cells.

Fatma Tosun,Esra Eroğlu Özkan,John A Beutler,Mahmut Miski,Feyyaz Mıhoğlugil

doi:10.3390/molecules25133040

Abstract

Several simple and prenylated coumarin derivatives were isolated from the dichloromethane extract of the root of Neocryptodiscus papillaris based on moderate cytotoxic activity of the extract in COLO205, KM12 and MCF7 cancer cells. While the major prenylated furanocoumarin derivatives and osthol isolated from the dichloromethane extract were responsible for the activity in the colon and breast cancer cell lines, the 4′-acylated osthol derivatives including a novel coumarino-alkaloid; neopapillarine) demonstrated selective cytotoxic activity in A498 and UO31 renal cancer cell lines.

Highlights

The flora of Turkey is highly diverse, with about seed plant species, 3100 of which are endemic [1].As part of a long-term collaboration to explore the phytochemistry of rare, endemic, and medicinal Turkish plants, we evaluated the anticancer properties of extracts and pure compounds using cancer cell growth inhibition assays as well as molecularly targeted assays
Apiaceae were tested by both cell growth and biochemical assays at the Molecular Targets Program, National Cancer Institute (NCI)
Neocryptodiscus [3] is a small genus of the family Apiaceae represented by five species worldwide [4], which have not been investigated for their non-volatile secondary metabolites

Summary

Introduction

The flora of Turkey is highly diverse, with about seed plant species, 3100 of which are endemic [1]. As part of a long-term collaboration to explore the phytochemistry of rare, endemic, and medicinal Turkish plants, we evaluated the anticancer properties of extracts and pure compounds using cancer cell growth inhibition assays as well as molecularly targeted assays. We have focused on the family Apiaceae, a notably diverse plant family in Turkey, with 100 genera, e.g., [2]. Neocryptodiscus [3] is a small genus of the family Apiaceae represented by five species worldwide [4], which have not been investigated for their non-volatile secondary metabolites. Prangos papillaris (Boiss.) Menemen) is a rare species only found in a small area of the Northern Mesopotamia region [5]

Methods

Results

Conclusion