Investigation of in vitro cytotoxic activity of pigment extracted from Salinicoccus sp. isolated from Nellore sea coast

Abstract

Objective: To investigate the in vitro cytotoxic activity of pigment extracted from Salinicoccus sp. isolated from Nellore sea coast. Materials and Methods: In the present study, pigment-forming bacteria were isolated from samples collected from Nellore coast, Andhra Pradesh. Among different pigmented isolates obtained on Zobell agar medium, the pinkish orange bacterium was selected for the study. The bacterium was cultured in Zobell broth medium and incubated in an orbital shaker at 120 rpm for 6 days at 25°C. After incubation, the culture broth was centrifuged at 10,000 rpm for 10 min to obtain a pellet, and the pellet was extracted using the solvent methanol and acetone (5:1). The crude pigment extract was evaluated for cytotoxic potential and was found to exhibit cytotoxic effect on A549 (human lung cancer) and MCF-7 (breast cancer) cell lines. The cell lines A549 cells, MCF-7, were cultured in Dulbecco's Modified of Eagle medium with L-glutamine and 1000 mg/L glucose supplemented with 10% fetal bovine serum, penicillin G (100 units/ml), and streptomycin sulphate (0.1 mg/ml) in a humidified atmosphere consisting of 5% CO2at 37°C. Results: The results of the present study revealed that the crude pigment extract has a strong anticancer potential, especially toward the A549 (Lung cancer cell lines) and MCF-7 (Breast cancer cell lines), respectively. Conclusion: This study clearly indicated that the pigment extract of marine Salinicoccus sp. has a strong cytotoxic activity against A549 (lung) and MCF-7 (breast) cancer cell lines which may be utilized for the drug development.