Abstract
This data article restrains data associated to the Choudhary et al. [1]. Nemopilema nomurai Jellyfish venom (NnV) can lead to cardiac toxicity. Here we analyzed the effect of NnV on rat cardiomyocytes cell line H9c2 at the proteome level using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). This analysis resulted in 34 proteins with differential expression. Here we provide the dataset for the proteins with amplified or reduced level as compare to control.
Highlights
This data article restrains data associated to the Choudhary et al [1]
We analyzed the effect of Nemopilema nomurai Jellyfish venom (NnV) on rat cardiomyocytes cell line H9c2 at the proteome level using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS)
Table Analysis of the 2-DE gels using Progenesis Same Spots software, protein identification by MALDI-TOF/MS Analyzed To analyze NnV-related proteomic changes, H9c2 cells incubated in the absence or the presence of 3 mg/ml NnV for 6 and 12 h After treatment, 2-DE gels were run and analyzed using Progenesis Same Spots software and identified by MALDI-TOF/MS Specimens of N. nomurai jellyfish were collected from Yellow Sea along the coast of Gunsan, South Korea
Summary
Specimens of N. nomurai jellyfish were collected from Yellow Sea along the coast of Gunsan, South Korea. Nematocysts were isolated as described previously [2]. To remove any debris, dissected tentacles were rinsed with cold sea water, and 3 volumes (v/v) of cold sea water was added and kept on shaker for 1 day at 4 °C. After that tentacle free sea water was centrifuged at 1000g for 5 min, pellet was collected and washed thrice with sea water. Sedimented tentacles were further autolysed in fresh sea water at 4 °C for 1 day as described above and autolysis process was repeated for 3–4 days. The settled nematocysts were collected and washed several times with sea water. Nematocysts were harvested at 500g for 5 min and pellets (nematocysts) were lyophilized and stored at À 20 °C until further use
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