Abstract
The rice root-knot nematode Meloidogyne graminicola has emerged as a devastating pest of rice in South-East Asian countries. Here we present a draft genome sequence for M. graminicola , assembled using data from short and long insert libraries sequenced on Illumina GAIIx sequencing platform.
Highlights
Considering the ~30 Mb genome size of M. graminicola as predicted by Feulgen densitometry (Lapp and Triantaphyllou, 1972), we planned to generate two libraries of varying insert length with ~150× depth of data (~4.5 Gb) per library using paired-end sequencing to achieve a comprehensive assembly
Hatched second stage juveniles were used for the genomic DNA extraction using Gentra Puregene Tissue Kit (Cat No.: 158667 Qiagen, Valencia, CA, USA)
The sequencing was performed on Illumina GAIIx platform at the Genotypic Technology Pvt
Summary
Considering the ~30 Mb genome size of M. graminicola as predicted by Feulgen densitometry (Lapp and Triantaphyllou, 1972), we planned to generate two libraries of varying insert length with ~150× depth of data (~4.5 Gb) per library using paired-end sequencing to achieve a comprehensive assembly. We report the sequencing and assembly of the genome of M. graminicola IARI strain. The secondary assembly was further refined by Redundans pipeline (Pryszcz and Gabaldón, 2016) to generate the final genome assembly with a minimum sequence length of 500 bp.
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