Negative Pharmacokinetics Evaluation of Liquorice and Pomegranate Juices on Potential of Salbutamol in Rats

Abstract

Objectives: Beverages or food may alter the pharmacokinetic parameters and pharmacodynamics of drugs-substances’ active pharmaceutical ingredients which may cause adverse circumstances. Salbutamol is widely indicated to prevent asthma and acute/chronic obstructive pulmonary disorders. Liquorice and/or Pomegranate are prescribed to support cough and asthma management. Nonetheless, an interaction thereafter with a Salbutamol pharmacokinetics was not investigated yet. Thus, the aim of this research is to evaluate the effect of Liquorice and/or Pomegranate on Salbutamol pharmacokinetic-parameters in animals (rats). Methods and Materials: A Chromatography method of (HPLC) was carried out. Partially validated after a development profitably conformed to the European Medical Agency’s guidelines to measure Salbutamol in rat serum. Liquorice and/or Pomegranate juices were given to rats for three days and, an hour sooner than Salbutamol (5.0 mg/kg), it was administered as an oral solution. Blood samples were collected at scheduled intervals, then handled and investigated by HPLC methodology. Results: The linearity of the calibration curve was over 98%. The intra-day and the inter-day accuracy values of Salbutamol were (97.93%-105.18%), and (98.67%-105.91%), respectively. While the intra- and inter-day precision values were equal to or less than 3.178% and 3.653%, respectively. In addition, no significant change was seen in the pharmacokinetic parameters of Salbutamol when administered to rats that were given either pomegranate or Liquorice juice. Conclusions: It was established that the given Liquorice or pomegranate juices with the above-mentioned orally administrated Salbutamol solution, did not result in a significant parameter in the pharmacokinetic interaction.