Abstract
Ferulic acid esterases (FAEs) are promising enzymes for potential application in agricultural, food, paper, and pharmaceutical industries. Discovery and characterization of novel FAEs is still up to date. Depending on their substrate specificity towards four methyl hydroxycinnamates (methyl esters of ferulic, p-coumaric, caffeic, and sinapic acids), FAEs are classified into four subgroups, named A–D types. In this work we investigate practical applicability of near UV difference spectroscopy for FAE’s substrate specificity determination. Difference spectroscopy is demonstrated to be a convenient and easily performed approach, enabling either single point or continuous determination of four methyl hydroxycinnamates hydrolysis rate at a concentration range 0.02–1.0mM. Difference maxima wavelengths and corresponding extinction coefficients are determined for free and methyl esterified ferulic, p-coumaric, caffeic, and sinapic acids.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
