Abstract
A colorimetric near-infrared fluorescent probe was designed and synthesized in this paper. The probe XCCC was obtained by simple condensation of 6-(diethylamino)-2,3-dihydro-1H-xanthene-4-carbaldehyde and 3-cyanoacetyl indoles. XCCC detects HSO3− through 1, 4-nucleophilic addition reaction, with obvious near-infrared fluorescence quenching (λem = 696 nm), and the solution color changes from pink to pale yellow under natural light to realize naked eye recognition. In addition, XCCC has a super large Stokes shift value (196 nm) by calculation. The sensitivity of XCCC and HSO3− in the concentration range of 0 ∼ 60 μM is excellent, and the LOD value is as low as 11.4 nM. It is very fast for XCCC to detect HSO3− in real time (30 s), and the reversible cycle of HSO3−- H2O2 is realized (5 times). In practical application, XCCC successfully detected HSO3− qualitatively and quantitatively in actual water samples. Furthermore, we made XCCC into fluorescent test strips to qualitatively detect HSO3− in red wine. In particular, XCCC was used to realize exogenous cell imaging of HSO3− with different concentrations and reversible cell imaging of HSO3−-H2O2 in A549 cells. It shows that probe XCCC has a potential application prospect in food samples and life systems.
Published Version
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