Near-Complete Genome Sequence of a Newly Emerging Subgenotype of Atypical Porcine Pestivirus.

Xiaoru Wang,Dongsheng He,He Yan,Yongsheng Xie,Kenneth M Stedman

doi:10.1128/mra.00115-20

Xiaoru Wang, Dongsheng He + Show 3 more

Open Access

https://doi.org/10.1128/mra.00115-20

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Abstract

This study reports the near-complete genome sequence of GD-CT4, a strain of a newly emerging subgenotype of atypical porcine pestivirus detected in a newborn piglet with congenital tremors in Guangdong Province, China. This sequence will improve the understanding of the epidemiology and genetic characteristics of atypical porcine pestivirus.

Highlights

This study reports the near-complete genome sequence of GD-CT4, a strain of a newly emerging subgenotype of atypical porcine pestivirus detected in a newborn piglet with congenital tremors in Guangdong Province, China
The GD-CT4 sequence was 11,101 bp long, and its GϩC content was 46.0%, obtaining 96.5% of the genome relative to the reference sequence (GenBank accession number MK216752)
A complete open reading frame (ORF) (10,908 bp) encoding a polyprotein of 3,635 amino acids was found between nucleotide positions 45 and 10952

Summary

Introduction

This study reports the near-complete genome sequence of GD-CT4, a strain of a newly emerging subgenotype of atypical porcine pestivirus detected in a newborn piglet with congenital tremors in Guangdong Province, China. APPV is known as a highly variable single-stranded RNA virus with an ϳ11- to 12-kb genome that contains a long open reading frame (ORF) encoding a polyprotein of four structural proteins (C, Erns, E1, and E2) and eight nonstructural proteins (Npro, P7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B) [1]. The GD-CT4 sequence was 11,101 bp long, and its GϩC content was 46.0%, obtaining 96.5% of the genome relative to the reference sequence (GenBank accession number MK216752).

Results

Conclusion