NcRPI-LGAT: Prediction of ncRNA-protein interactions with line graph attention network framework

Abstract

Identification of ncRNA-protein interactions (ncRPIs) through wet experiments is still time-consuming and highly-costly. Although several computational approaches have been developed to predict ncRPIs using the structure and sequence information of ncRNAs and proteins, the prediction accuracy needs to be improved, and the results lack interpretability. In this work, we proposed a novel computational method (called ncRPI-LGAT) to predict the ncRNA-Protein Interactions by transforming the link prediction (i.e., subgraph classification) task into a node classification task in the line network, and introducing a Line Graph ATtention network framework. ncRPI-LGAT first extracts the ncRNA/protein attributes using node2vec, and then generates the local enclosing subgraph of a target ncRNA-protein pair with SEAL. Because using the pooling operations in local enclosing subgraphs to learn a fixed-size feature vector for representing ncRNAs/proteins will cause the information loss, ncRPI-LGAT converts the local enclosing subgraphs into their corresponding line graphs, in which the node corresponds to the edge (i.e., ncRNA-protein pair) of the local enclosing subgraphs. Then, the attention mechanism-based graph neural network GATv2 is used on these line graphs to efficiently learn the embedding features of the target nodes (i.e., ncRNA-protein pairs) by focusing on learning the significance of one ncRNA-protein pair to another ncRNA-protein pair. These embedding features of one ncRNA-protein pair obtained from multi-head attention are concatenated in series and then fed them into a fully connected network to predict ncRPIs. Compared with other state-of-the-art methods in the 5CV test, ncRPI-LGAT shows superior performance on three benchmark datasets, demonstrating the effectiveness of our ncRPI-LGAT method in predicting ncRNA-protein interactions.