Abstract
Precise manipulation of micro and nanosized particles has enabled investigations into various applications ranging from mechanobiology of biomolecules and cells to self-assembly of two-dimensional colloids. This work is focussed on studying the nature of a noninvasive electrothermal vortex based micro-manipulation tool called rapid electrokinetic patterning (REP). Using the equipartition method, we show that a REP trap is Hookean in nature and has an ultralow trap stiffness on the order of femtonewtons/\ensuremath{\mu}m. The dynamic tunability of an optically induced REP trap makes it a versatile tool for various biophysical applications.
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