Naturally occurring protein/polysaccharide hybrid nanoparticles for stabilizing oil-in-water Pickering emulsions and the formation mechanism

Abstract

In this study, we reported for the first time that the natural protein/polysaccharide hybrid nanoparticles (PPH NPs) with a diameter of ∼ 129 nm, originating from Lactobacillus plantarum fermented cheese whey, could act as green-based NPs for stabilizing Pickering emulsions. Characterizations of PPH NPs showed that the negative-charged PPH NPs were composed of ∼ 37.7% total protein and ∼ 7.3% polysaccharide bearing several functional groups, such as –OH, –NH, –COOH, etc.; and displayed excellent emulsifying capacity in preparing oil-in-water Pickering emulsions. The obtained emulsions exhibited gel-like behavior with excellent stability against the variation of pH, ionic strength, and temperature. Confocal observations showed that PPH NPs effectively adsorbed and anchored at the oil–water interface, thus creating the steric hindrance to inhibit droplet coalescence. This research is of importance in developing novel and biocompatible Pickering stabilizers with outstanding performance, as well as enable a versatile design of stable Pickering emulsions suitable for food industries.