Natural priming as an important metabolic event in the life history of Wigandia urens (Hydrophyllaceae) seeds

Abstract

Natural priming promotes fast and synchronic seed germination and enhances the establishment of seedlings from Wigandia urens seeds. In this study, seeds of this species were buried in the field in three different sites, and each site was divided into three different microsites (natural priming). They were then exhumed and air‐dried at the beginning of the rainy season. The control and exhumed W. urens seeds were tested for differences in germination, and in the protein patterns derived from heat‐stable and phospho‐protein enriched fractions, sucrose concentrations, amylase isoenzyme patterns, as well as catalase activity and H2O2 levels. All the exhumed seeds germinated faster and more synchronically than the control seeds. Nevertheless, the germinative pattern showed a microsite effect related to the microenvironmental conditions. Most exhumed samples kept the germinative advantages acquired from the soil during natural priming for 2 years but seeds from the most heterogeneous site (a forest gap) did not. Natural priming also promoted mobilization of 7‐S globulin‐like proteins, solubilization of the 11‐S globulin‐like proteins as well as sucrose consumption before radicle protrusion. Although there were no changes in starch concentration during the germination of either the control or buried seeds, a different isoenzyme amylase pattern was observed. Catalase activity decreased and hydrogen peroxide levels were lower in exhumed seeds during germination. The changes in the protein and sucrose patterns were related to advances in the germinative process acquired during burial. Metabolic advantages were maintained systematically in all seed samples along the 2 years. The ecological significance of natural priming is discussed.