Natural organic matters promoted conjugative transfer of antibiotic resistance genes: Underlying mechanisms and model prediction

Abstract

Dissemination of antibiotic resistance gene (ARG) is a huge challenge around the world. Natural organic matter (NOM) is one of the most commonly components in aquatic systems. Information regarding ARG transfer induced by NOM is still lacking. In this study, experimental exploration and model prediction on RP4 plasmid conjugative transfer between bacteria under NOM exposure was conducted. Compared with no exposure, the conjugative transfer frequency of RP4 plasmid increased 7.1-fold and 3.2-fold under exposure to 10 kDa and 100 kDa NOM exposure, respectively. NOM exposure with a lower molecular weight and higher concentration promoted gene expressions related to reactive oxygen species generation, cell membrane permeability, intercellular contact, quorum sensing, and energy driving force. Concurrently, the expressions of conjugation genes in RP4 plasmid were also upregulated. Moreover, model prediction demonstrated that the maintenance of the acquired plasmid was shortened to 133 h under 10 kDa NOM exposure compared with the control (200 h). Long-term NOM exposure enhanced transfer frequency and transfer rate of ARG. This study firstly theoretically and experimentally revealed the underlying mechanisms for promoting ARG transfer by NOM.