Abstract
9605 Background: NK cells express killer immunoglobulin receptors (KIR) that recognize certain HLA class I ligands, thus preventing them from killing those cells. In haploidentical transplantation for leukemia, mismatch between donor and recipient KIR ligands correlated with a remarkably low relapse rate. We investigated if antibody-dependent cell cytotoxicity (ADCC) mediated by allogeneic NK cells against the HER2+ cell line, SKBR3, is affected by KIR ligand incompatibility. Furthermore, we investigated if KIR inhibition could be overcome by trastuzumab. Methods: Genotyping established that donor 1 and the SKBR3 cell line belonged to the C1 group, whereas donor 2 was in the C2 group. KIR genotyping established that donor 2 had all of donor 1's KIR genes, plus the inhibitory 2DL5A and the activating 2DS3 and 3DS1 genes. Donor NK cells were isolated using a magnetic bead isolation kit, and NK cell cytotoxicity was assessed by flow cytometry. In some experiments, trastuzumab (50ug/ml) was added to the targe...
Published Version
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